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KMID : 0985420110330010043
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Laboratory Medicine and Quality Assurance
2011 Volume.33 No. 1 p.43 ~ p.47
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Evaluation of two Real-Time PCR Assays for the Detection of Chlamydia trachomatis
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Lee Eun-Young
Kim Sin-Young
Kim Hyon-Suk
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Abstract
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BackgroundChlamydia trachomatis is common etiological agent of sexual transmitted disease. We evaluated two real-time PCR assays for the detection of C. trachomatis; Cobas TaqMan CT test v2.0 (Roche Diagnostics, Switzerland) and AdvanSure CT/NG real-time PCR (LG Life Sciences, Korea). And we compared performance of two real-time PCR assays with Cobas Amplicor CT test (Roche Diagnostics, Switzerland).
Methods:Seventy-nine cervical swab specimens collected from patients with suspected C. trachomatis infection were tested using CTM CT, ADVANSURE, and AMPLICOR for the detection of C. trachomatis.
Results:Twenty-five (31.6%) samples showed positive results in all three assays. The positive and negative concordance rates among three assays were 100%
Conclusion:CTM CT and ADVANSURE showed excellent agreement with AMPLICOR. Therefore, these assays were reliable for the evaluation of C. trachomatis infection.
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KEYWORD
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Chlamydia trachomatis, Real-time polymerase chain reaction, Comparison
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